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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD126/IL-6R alpha Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 62.5-4000 pg/mL

Cat No : MP00182-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00182-2, Mouse CD126/IL-6R alpha Monoclonal Matched Antibody Pair - PBS only. 98024-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0664. 83209-2-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

    Sandwich ELISA standard curve of MP00182-2

    Sandwich ELISA standard curve of MP00182-2, Mouse CD126/IL-6R alpha Monoclonal Matched Antibody Pair - PBS only. 98024-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0664. 83209-2-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

  • Serum of sixteen mice were measured. The IL-6R alpha concentration of detected samples was determined to be 10,180.3 pg/mL with a range of 6,065.2-15,362.2 pg/mL.

    Sample test of MP00182-2

    Serum of sixteen mice were measured. The IL-6R alpha concentration of detected samples was determined to be 10,180.3 pg/mL with a range of 6,065.2-15,362.2 pg/mL.

  • J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 106 cells/mL) were cultured for 3 days in DMEM containing 10% fetal bovine serum. An aliquot of the cell culture supernatants were removed, assayed for mouse IL-6R alpha, and measured 402.5 pg/mL. Liver tissue from one mouse was homogenized and seeded into 100 mL of RPMI containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and 50 μM β-mercaptoethanol for 3 days. An aliquot of the cell culture supernate was removed, assayed for mouse IL-6R alpha, and measured 345.1 pg/mL.

    Sample test of MP00182-2

    J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 106 cells/mL) were cultured for 3 days in DMEM containing 10% fetal bovine serum. An aliquot of the cell culture supernatants were removed, assayed for mouse IL-6R alpha, and measured 402.5 pg/mL. Liver tissue from one mouse was homogenized and seeded into 100 mL of RPMI containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and 50 μM β-mercaptoethanol for 3 days. An aliquot of the cell culture supernate was removed, assayed for mouse IL-6R alpha, and measured 345.1 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00182-2 targets CD126/IL-6R alpha in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 62.5-4000 pg/mL (Sandwich ELISA);

Capture antibody: Anti-Mouse CD126/IL-6R alpha Rabbit Recombinant Antibody, PBS Only 98024-1-PBS (230371A7). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Mouse Il6ra Recombinant antibody, PBS Only (Capture/Detector) 83209-2-PBS (230371E7). 100 μg. Concentration 1 mgl/ml.

Alternative CD126/IL-6R alpha matched antibody pairs: MP00182-1, MP00676-1, MP00676-2, MP50060-1

Matched pair protein standard: Eg0664

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 98024-1-PBS
Tested Reactivity mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 receptor, alpha
Synonyms Il6ra, CD126, 230371A7, IL-6R alpha, Il6r
GenBank accession numberNM_010559.3
Gene symbol Il6ra
UniprotP22272
Gene ID (NCBI) 16194
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purfication
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83209-2-PBS
Tested Reactivity mouse
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 receptor, alpha
Synonyms CD126, 230371E7, IL-6Rα, IL-6R alpha, Il6r
GenBank accession numberNM_010559.3
Gene symbol Il6ra
UniprotP22272
Gene ID (NCBI) 16194
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.