Mouse IL-13 ELISA Kit
Cat no : KE10021
Synonyms
Il 13, Il13, IL-13, interleukin 13, Interleukin-13, T-cell activation protein P600
Validation Data Gallery
Product Information
KE10021 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-13 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-13. The assay recognizes mouse IL-13. An antibody specific for mouse IL-13 has been pre-coated onto the microwells. The mouse IL-13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-13 is added to detect the captured mouse IL-13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-13 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16163 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 98% | 86%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 20.9 | 2.0 | 9.7 |
| 2 | 20 | 223.1 | 14.1 | 6.3 |
| 3 | 20 | 942.7 | 55.5 | 5.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 63.6 | 5.1 | 8.0 |
| 2 | 24 | 264.1 | 12.5 | 4.7 |
| 3 | 24 | 868.6 | 45.6 | 5.3 |
Background Information
Interleukin 13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. IL-13 up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. IL-13 is thought to be important cytokine in the pathogenesis of asthma, and more recently has been shown to play a pivotal role in a number of fibrotic diseases including hepatic and pulmonary fibrosis, and nodular sclerosing Hodgkin's disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il 13, Il13, IL-13, interleukin 13, Interleukin-13, T-cell activation protein P600 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Biomed Pharmacother Aster tataricus attenuates asthma efficiently by simultaneously inhibiting tracheal ring contraction and inflammation. | ||
Basic Clin Pharmacol Toxicol SIRT1/IGFBPrP1/TGF β1 axis involved in cucurbitacin B ameliorating concanavalin A-induced mice liver fibrosis. | ||
Biosci Biotechnol Biochem Lactobacillus helveticus SBT2171 alleviates allergic symptoms in a murine model for pollen allergy. | ||
Immunol Lett Role of G-protein-coupled estrogen receptor in the pathogenesis of chronic asthma |