Mouse IL-21 ELISA Kit

Sensitivity

0.5 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Mouse

Cat no : KE10012

Synonyms

Il21, IL-21, interleukin 21, Interleukin-21



Product Information

KE10012 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL-21 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-21. The assay recognizes mouse IL-21. An antibody specific for mouse IL-21 has been pre-coated onto the microwells. The mouse IL-21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-21 is added to detect the captured mouse IL-21 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-21 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.5 pg/mL
Range 15.6-1000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 60505

Recovery

Sample TypeAverageRange
Mouse serum92%77%-104%
Cell culture supernatants76%71%-86%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12074.74.86.5
220183.612.46.7
320717.538.45.4

InterAssay

Samplenmean ( pg/mL)SDCV%
12477.76.98.9
224198.39.44.7
324941.589.49.5

Background Information

Interleukin 21 (IL-21) is a cytokine produced predominantly by cluster of differentiation 4 (CD4+) T-cells and natural killer T-cells. IL-21 is preliminarily expressed in activated cluster of differentiation 4 (CD4+) T-cells and natural killer (NK) T-cells. IL-21 receptor (il21R) is a class I cytokine receptor, which requires dimerization with the indispensable common gamma chain (γc) subunit to bind IL-21. IL-21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. IL-21 mediates apoptosis in B-CLL cells through up-regulation of the BIM (BH3 family member) and enhances both direct and antibody-dependent cellular cytotoxicity in these cells.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il21, IL-21, interleukin 21, Interleukin-21

Publications

SpeciesSample TypeTitle
mouseserum

Biosci Biotechnol Biochem

Interleukin-21 knockout reduces bone loss in ovariectomized mice by inhibiting osteoclastogenesis

Authors - Junlong Hou
mousecell culture supernatants

Int Immunopharmacol

CIRP increases Foxp3+ regulatory T cells and inhibits development of Th17 cells by enhancing TLR4-IL-2 signaling in the late phase of sepsis

Authors - Zongsheng Wu