Mouse IL-22 ELISA Kit
Cat no : KE10041
Synonyms
IL 22, IL TIF, Il22, IL-22, Iltif, interleukin 22
Validation Data Gallery
Product Information
KE10041 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL22 ELISA kit is to be used to detect and quantify protein levels of endogenous IL22. The assay recognizes mouse IL22. An antibody specific for IL22 has been pre-coated onto the microwells. The IL22 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL22 is added to detect the captured mouse IL22 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-22 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 10.2 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 50929 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 87% | 75%-110% |
| Cell culture supernatants | 102% | 85%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 978.7 | 32.8 | 3.4 |
| 2 | 20 | 499.8 | 18.4 | 3.7 |
| 3 | 20 | 252.7 | 5.6 | 2.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 996.4 | 40.2 | 4.0 |
| 2 | 24 | 488.9 | 26.8 | 5.5 |
| 3 | 24 | 253.8 | 8.9 | 3.5 |
Background Information
Interleukin-22 (IL-22) is a recently described IL-10 family cytokine that is produced by T helper (Th) 17 cells, γδ T cells, NKT cells, and newly described innate lymphoid cells (ILCs). IL-22 has the potential to interact with IL-10 because it binds to the IL-10R2c chain with IL-22R1 in its receptor complex. Binding can be blocked by the soluble receptor, IL-22 binding protein (IL-22BP). IL-22 primarily targets nonhematopoietic epithelial and stromal cells, where it can promote proliferation and play a role in tissue regeneration. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL 22, IL TIF, Il22, IL-22, Iltif, interleukin 22 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Front Immunol E. coli Nissle 1917 ameliorates mitochondrial injury of granulosa cells in polycystic ovary syndrome through promoting gut immune factor IL-22 via gut microbiota and microbial metabolism |