Validation Data Gallery
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Tested Applications
| Positive WB detected in | |
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IP | See 1 publications below |
| FC | See 1 publications below |
Product Information
66360-2-Ig targets Mouse IgG2a isotype control in WB, FC , IP, ELISA applications and shows reactivity with megathura crenulata samples.
| Tested Reactivity | megathura crenulata |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | Mouse IgG2a isotype control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881739 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Mouse IgG2a isotype control antibody 66360-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biosens Bioelectron Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes. | ||
Nat Commun Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire | ||