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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse IgG2b isotype control Monoclonal antibody, PBS Only

Mouse IgG2b isotype control Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

megathura crenulata

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

11B8C4

Cat no : 66360-3-PBS

Synonyms

Mouse IgG2b

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig)

    WB analysis using 66360-3-Ig (same clone as 66360-3-PBS)

    Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig)

    IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig)

    IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig)

    FC experiment of human PBMCs using 66360-3-Ig (same clone as 66360-3-PBS)

    1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig, Clone: 11B8C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Anti-Human CD20 (65085-1-Ig) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (green), or unstained (blue). Cells were not fixed. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66360-3-PBS targets Mouse IgG2b isotype control in WB, IHC, ELISA applications and shows reactivity with megathura crenulata samples.

Tested Reactivity megathura crenulata
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Mouse IgG2b isotype control
Gene symbol
Gene ID (NCBI)
RRIDAB_2881740
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).