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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mouse IgG3 isotype control Monoclonal antibody (66360-4-Ig)

    WB analysis using 66360-4-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-4-Ig (KLH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Mouse IgG3 isotype control Monoclonal antibody

Mouse IgG3 isotype control Monoclonal Antibody for WB, ELISA
Cat No. 66360-4-Ig
Clone No.1H4A5

Host / Isotype

Mouse / IgG3

Reactivity

Megathura crenulata

Applications

WB, ELISA

Synonyms

IgG3 isotype, mouse IgG control, Mouse IgG3 control, Mouse IgG3 isotype control

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66360-4-Ig targets Mouse IgG3 isotype control in WB, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Mouse IgG3 isotype control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2923630
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG3. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Protocols

Product Specific Protocols
WB protocol for Mouse IgG3 isotype control antibody 66360-4-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols