Mouse Lineage Depletion Kit
Cat no : KMS301
Validation Data Gallery
Product Information
Mouse Lineage Depletion Kit is designed to deplete major hematopoietic lineage-committed cells from mouse bone marrow cells. After lineage cell depletion, c-kit+ mouse hematopoietic progenitor cells will be enriched. This kit contains two components: biotin labeled mouse lineage antibody cocktail and streptavidin magnetic beads. Through incubation of samples with biotin antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled lineage-committed cells will be retained in the tube by magnetic separator. The untouched mouse hematopoietic progenitor cells are collected in supernatant. Any downstream application can be performed on hematopoietic progenitor cells after separation, such as Flow Cytometry, transfection and in vitro culture.
Components
KMS301-10:
· MS301A-10: 300 µL biotin labeled mouse lineage antibody cocktail (Ter119, Gr-1, CD11b, CD45R, CD3)
· MS301B-10: 300µL streptavidin magnetic beads
KMS301-100:
· MS301A-100: 3mL biotin labeled mouse lineage antibody cocktail (Ter119, Gr-1, CD11b, CD45R, CD3)
· MS301B-100: 3mL streptavidin magnetic beads
Storage
2-8°C
Storage buffer
PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide
Reactivity
Mouse
Recommend usage
30µL biotin labeled antibody cocktail and 30µL streptavidin beads for 1*107 total mouse bone marrow cells.
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sara (Verified Customer) (12-10-2024) | Easy to use. B220 (CD45R) depletion could be improved, about 30% B220+ cells remain after isolation (after one round, see image). Subsequent rounds can be used to purify further. It works to enrich c-kit+ cells.
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FH Cindy (Verified Customer) (09-06-2024) | n/a
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