Mouse NGAL ELISA Kit
Cat no : KE10045
Synonyms
24p3, AW212229, Lcn2, lipocalin 2, NGAL
Validation Data Gallery
Product Information
KE10045 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous mouse NGAL. The assay recognizes mouse NGAL. An antibody specific for mouse NGAL has been pre-coated onto the microwells. The mouse NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse NGAL is added to detect the captured mouse NGAL protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse NGAL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates, Urine |
| Assay type | Sandwich |
| Sensitivity | 4.0 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16819 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 106% | 95%-120% |
| Mouse urines | 116% | 92%-131% |
| Cell culture supernates | 106% | 79%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 429.5 | 33.2 | 7.7 |
| 2 | 20 | 109.1 | 4.7 | 4.3 |
| 3 | 20 | 26.6 | 2.0 | 7.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 486.3 | 40.4 | 8.3 |
| 2 | 24 | 114.7 | 6.3 | 5.5 |
| 3 | 24 | 20.5 | 1.7 | 8.5 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL; also known as neutrophil lipocalin, lipocalin-2, siderocalin, 24p3, or LCN2) is a small molecule of almost 25 kd that belongs to the well-defined superfamily of proteins called lipocalins. NGAL initially found in activated neutrophils, in accordance with its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 24p3, AW212229, Lcn2, lipocalin 2, NGAL |
Publications
| Species | Sample Type | Title |
|---|---|---|
Phytomedicine Dihydromyricetin attenuates intracerebral hemorrhage by reversing the effect of LCN2 via the system Xc- pathway |