Mouse/Rat PDGF-AB ELISA Kit
Cat no : KE10057
Synonyms
PDGF AB, Pdgfa, PDGF-AB
Validation Data Gallery
Product Information
KE10057 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat PDGF-AB ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat PDGF-AB. The assay recognizes mouse/rat PDGF-AB. An antibody specific for mouse/rat PDGF-AB has been pre-coated onto the microwells. The mouse/rat PDGF-AB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat PDGF-AB is added to detect the captured mouse/rat PDGF-AB protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat PDGF-AB ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Mouse serum, Mouse plasma, Rat serum, Rat plasma |
| Assay type | Sandwich |
| Sensitivity | 3.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18590 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 83% | 76%-97% |
| Rat serum | 85% | 76%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 492.4 | 14.1 | 2.9 |
| 2 | 20 | 108.6 | 2.0 | 1.8 |
| 3 | 20 | 25.1 | 1.1 | 4.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 501.5 | 5.2 | 1.0 |
| 2 | 24 | 119.6 | 1.7 | 1.4 |
| 3 | 24 | 28.1 | 2.3 | 8.3 |
Background Information
Platelet-derived growth factor (PDGF) is one of the most abundant growth factors contained in the platelet alpha-granules and released upon platelet activation. It is also secreted by epithelial and mesenchymal cells. PDGF family consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. The PDGFR receptors are encoded by two genes: PDGFRA and PDGFRB. PDGFR is known to control growth, cell movement, angiogenesis and embryo development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PDGF AB, Pdgfa, PDGF-AB |
Publications
| Species | Sample Type | Title |
|---|---|---|
Brain Sci Double Blast Wave Primary Effect on Synaptic, Glymphatic, Myelin, Neuronal and Neurovascular Markers |