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Multi-rAb HRP-Goat Anti-Mouse IgG-Specific Recombinant Secondary Antibody

Multi-rAb HRP-Goat Anti-Mouse IgG-Specific Recombinant Secondary Antibody is a mixture of precisely engineered recombinant monoclonal antibodies that bind to Mouse IgG and do not react with Mouse IgM or IgA.

Applications

ELISA, WB, Dot blot

Conjugate

HRP

Host

Goat

Reactivity

Mouse

Cat no : RGAM601

Validation Data Gallery

  • Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, left panel) and Beta-actin mouse monoclonal antibody (60008-1-Ig, right panel). Multi-rAb HRP-Goat Anti-Mouse IgG1-Specific Recombinant Secondary Antibody were used at 1:20000 for detection. The result shows that the secondary does not react with mouse IgM.

    Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, left panel) and Beta-actin mouse monoclonal antibody (60008-1-Ig, right panel). Multi-rAb HRP-Goat Anti-Mouse IgG1-Specific Recombinant Secondary Antibody were used at 1:20000 for detection. The result shows that the secondary does not react with mouse IgM.

  • Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, mouse IgG1 subclass, left panel) and Gelsolin mouse monoclonal antibody (66280-1-Ig, mouse IgA class, right panel). Multi-rAb HRP-Goat Anti-Mouse IgG1-Specific Recombinant Secondary Antibody were used at 1:20000 for detection. The result shows that the secondary does not react with mouse IgA.

    Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, mouse IgG1 subclass, left panel) and Gelsolin mouse monoclonal antibody (66280-1-Ig, mouse IgA class, right panel). Multi-rAb HRP-Goat Anti-Mouse IgG1-Specific Recombinant Secondary Antibody were used at 1:20000 for detection. The result shows that the secondary does not react with mouse IgA.

  • Indirect ELISA was performed by coating ag26747 (GPNMB fusion protein) at 70 ng/well, ag0297 (beta actin fusion protein), and ag17916 (Gelsolin fusion protein) at 140ng/well. Corresponding mouse monoclonal antibodies 66926-1-Ig (IgG class), 60008-1-Ig (IgM class), and 66280-1-Ig (IgA class) were used as primary antibodies at 0.1μg/mL. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Mouse IgG-Specific Recombinant Secondary Antibody (RGAM601) was used for detection. The result indicates that RGAM601 strongly binds to Mouse IgG and does not react with Mouse IgM or IgA.

    Indirect ELISA was performed by coating ag26747 (GPNMB fusion protein) at 70 ng/well, ag0297 (beta actin fusion protein), and ag17916 (Gelsolin fusion protein) at 140ng/well. Corresponding mouse monoclonal antibodies 66926-1-Ig (IgG class), 60008-1-Ig (IgM class), and 66280-1-Ig (IgA class) were used as primary antibodies at 0.1μg/mL. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Mouse IgG-Specific Recombinant Secondary Antibody (RGAM601) was used for detection. The result indicates that RGAM601 strongly binds to Mouse IgG and does not react with Mouse IgM or IgA.

Product Information

RGAM601 targets Mouse IgG in ELISA and Western blot. It can bind to Mouse IgG and does not react with Mouse IgM or IgA.

Concentration 1.0 mg/mL
Applications ELISA, WB, Dot blot
Host Species Goat
Species Reactivity Mouse
Physical State Liquid
Suggested Dilution Range 1:10,000-1:100,000 for ELISA
1:10,000-1:200,000 for western blotting with ECL substrates (1:10,000-1:40,000 is suggested for most systems).
Purity The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
Storage Buffer PBS with 50% glycerol, 10 mg/mL BSA, 0.1% Proclin-300, pH 7.4.
Storage Instructions Store at -20°C. Stable for one year after shipment.
ClonalityMulticlonal recombinant
RRIDAB_3662741
NoteThis product is for research use only, not for diagnostic or therapeutic use.

Background

Multi-rAb Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal mixture is carefully selected after rigorous characterization and screening to ensure the highest level of performance. ​The selected clones are mixed in various combinations, each of which is then functionally characterized in multiple applications to select the final multiclonal mixture with optimal performance. Each lot of Multi-rAb Secondary Antibodies is produced by mixing the same high-performing recombinant clones every time to ensure that they have high batch-to-batch consistency, which in turn results in high reproducibility of experimental data. This secondary antibody reacts with Mouse IgG but does not react with Mouse IgM or IgA.