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Multi-rAb Polymer HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L)

Multi-rAb Goat Anti-rabbit recombinant secondary antibody is a mixture of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on rabbit IgG heavy and light chain.

Applications

IHC

Conjugate

Polymer HRP

Host

Goat

Reactivity

Rabbit

Cat no : RGAR011

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP43 recombinant antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP43 recombinant antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (smooth muscle actin recombinant antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (smooth muscle actin recombinant antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (smooth muscle actin recombinant antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (smooth muscle actin recombinant antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin polyclonal antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin polyclonal antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin polyclonal antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin polyclonal antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin polyclonal antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin polyclonal antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using commercial IVD grade KI67 antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using commercial IVD grade KI67 antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using other vendor's NF-kappa B p65 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using other vendor's NF-kappa B p65 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using other vendor's BCL2 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using other vendor's BCL2 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer. Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer. Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using other vendor's ER rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using other vendor's ER rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21146-1-AP (LARS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21146-1-AP (LARS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21146-1-AP (LARS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21146-1-AP (LARS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Product Information

RGAR011 targets Rabbit IgG (H+L) in IHC. It is higly specific to rabbit IgG and shows minimal reactivity for mouse, rat, human, pig and bovine IgGs.

Concentration Ready to use
Applications IHC
Host Species Goat
Species Reactivity Rabbit
Physical State Liquid
Suggested Dilution Range Ready to use
Purity Protein G purification
Storage Buffer TBST+10 mg/mL BSA+1 mg/mL gelatin + 0.1% proclin300
Storage Instructions Store at 2-8°C. Stable for one year after shipment.
ClonalityMulticlonal recombinant
RRIDAB_3094534
NoteThis product is for research use only, not for diagnostic or therapeutic use.

Background

Multi-rAb Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal mixture is carefully selected after rigorous characterization and screening to ensure the highest level of performance. ​The selected clones are mixed in various combinations, each of which is then functionally characterized in multiple applications to select the final multiclonal mixture with optimal performance. Each lot of Multi-rAb Secondary Antibodies is produced by mixing the same high performing recombinant clones every time to ensure that they have high batch-to-batch consistency, which in turn results in high reproducibility of experimental data.
Multi-rAb Polymer HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) enables highly sensitive, biotin-free detection of rabbit primary antibodies in IHC. Unlike traditional avidin-biotin-based signal amplification methods, polymer HRP-based detection offers the advantages of a lower background and faster protocol with fewer steps.
Moreover, Multi-rAb Polymer HRP secondary antibodies are provided in a ready-to-use format, in easy-to-use dropper bottles making it extremely convenient for users to incorporate them into their IHC staining protocols.

Publications

Title

Drug Resist Updat

Lansoprazole (LPZ) reverses multidrug resistance (MDR) in cancer through impeding ATP-binding cassette (ABC) transporter-mediated chemotherapeutic drug efflux and lysosomal sequestration

Authors - Ning Ji
View Article

J Ethnopharmacol

Luteolin and its analog luteolin-7-methylether from Leonurus japonicus Houtt suppress aromatase-mediated estrogen biosynthesis to alleviate polycystic ovary syndrome by the inhibition of tumor progression locus 2

Authors - Xiao-Ke Shi
View Article

Microbiol Immunol

A fungal-binding agglutinin in the skin slime of Japanese flounder (Paralichthys olivaceus) is glyceraldehyde 3-phosphate dehydrogenase

Authors - Shigeyuki Tsutsui
View Article

Phytomedicine

Fructus Jujubae cooperated with water-expelling members in Shizao decoction alleviated intestinal injury and malignant ascites by modulating gut microbiota and metabolic homeostasis

Authors - Shi-Kang Zhou
View Article

Fish Shellfish Immunol

Grouper OTUB1 and OTUB2 promote Red-spotted grouper nervous necrosis virus (RGNNV) replication by inhibiting the host innate immune response

Authors - Siting Wu
View Article