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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human ATP1A1 (Polyclonal)

ATP1A1 Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

GAACATAATGATCCT

Cat no : G14418-1-5C

Synonyms

ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1

Validation Data Gallery

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  • Single Cell Sequencing experiment G14418-1-5C on Resting PBMC

    Single Cell Sequencing experiment G14418-1-5C on Resting PBMC

    G14418-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G14418-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G14418-1-5C on PBMC treated with 4hr LPS + TI

    G14418-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G14418-1-5C targets ATP1A1 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Immunogen ATP1A1 fusion protein Ag5763 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human ATP1A1 (Polyclonal)
Calculated molecular weight 113 kDa
GenBank accession numberBC050359
Gene symbol ATP1A1
Gene ID (NCBI) 476
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGAACATAATGATCCTCCCATATAAGAAA
Barcode SequenceGAACATAATGATCCT
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol