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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD11c (3.9)

CD11c Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Conjugate

5CFLX

Barcode Sequence

AAGCAATACTTATAC

CloneNo.

3.9

Cat no : G65086-1-5C

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

Validation Data Gallery

Filter:
  • Single Cell Sequencing experiment G65086-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65086-1-5C on Resting PBMC

    G65086-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65086-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65086-1-5C on PBMC treated with 4hr LPS + TI

    G65086-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65086-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65086-1-5C on Resting PBMC

    G65086-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65086-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65086-1-5C on PBMC treated with 4hr LPS + TI

    G65086-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65086-1-5C targets CD11c in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Immunogen Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD11c (3.9)
Calculated molecular weight 1169 aa, 129 kDa
GenBank accession numberBC038237
Gene symbol CD11c
Gene ID (NCBI) 3687
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGAAGCAATACTTATACCCCATATAAGAAA
Barcode SequenceAAGCAATACTTATAC
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol