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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD122/IL-2RB (Polyclonal)

CD122/IL-2RB Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

GGTCTGTGACAGCGA

Cat no : G13602-1-5C

Synonyms

CD122, CD122/IL-2RB, IL 2 receptor subunit beta, IL 2R subunit beta, IL 2RB, IL2RB, IL-2RB, interleukin 2 receptor, beta, P70 75, p75

Validation Data Gallery

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  • Single Cell Sequencing experiment G13602-1-5C on Resting PBMC

    Single Cell Sequencing experiment G13602-1-5C on Resting PBMC

    G13602-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G13602-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G13602-1-5C on PBMC treated with 4hr LPS + TI

    G13602-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G13602-1-5C targets CD122/IL-2RB in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Immunogen CD122/IL-2RB fusion protein Ag3929 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD122/IL-2RB (Polyclonal)
Calculated molecular weight 551 aa, 61 kDa
GenBank accession numberBC025691
Gene symbol IL2RB
Gene ID (NCBI) 3560
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGGTCTGTGACAGCGACCCATATAAGAAA
Barcode SequenceGGTCTGTGACAGCGA
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol