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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD127/IL-7R (Polyclonal)

CD127/IL-7R Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

CGGCTCGGACCATAG

Cat no : G17626-1-5C

Synonyms

CD127, CDW127, IL 7 receptor subunit alpha, IL 7R alpha, IL 7R subunit alpha, IL 7RA, IL7R, IL-7R, IL7RA, ILRA, interleukin 7 receptor

Validation Data Gallery

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  • Single Cell Sequencing experiment G17626-1-5C on Resting PBMC

    Single Cell Sequencing experiment G17626-1-5C on Resting PBMC

    G17626-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G17626-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G17626-1-5C on PBMC treated with 4hr LPS + TI

    G17626-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G17626-1-5C targets CD127/IL-7R in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Immunogen CD127/IL-7R fusion protein Ag11844 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD127/IL-7R (Polyclonal)
Calculated molecular weight 459 aa, 52 kDa
GenBank accession numberBC067537
Gene symbol CD127
Gene ID (NCBI) 3575
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCGGCTCGGACCATAGCCCATATAAGAAA
Barcode SequenceCGGCTCGGACCATAG
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol