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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD14 (2C1D9)

CD14 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1

Reactivity

Human

Conjugate

5CFLX

Barcode Sequence

ATCGTCTGTTGCGCG

CloneNo.

2C1D9

Cat no : G60253-1-5C

Synonyms

CD14, CD14 molecule

Validation Data Gallery

Filter:
  • Single Cell Sequencing experiment G60253-1-5C on Resting PBMC

    Single Cell Sequencing experiment G60253-1-5C on Resting PBMC

    G60253-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G60253-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G60253-1-5C on PBMC treated with 4hr LPS + TI

    G60253-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G60253-1-5C on Resting PBMC

    Single Cell Sequencing experiment G60253-1-5C on Resting PBMC

    G60253-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of G60253-1-5C is CD14 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Immunogen CD14 fusion protein Ag10693 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD14 (2C1D9)
Calculated molecular weight 375 aa, 40 kDa
GenBank accession numberBC010507
Gene symbol CD14
Gene ID (NCBI) 929
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGATCGTCTGTTGCGCGCCCATATAAGAAA
Barcode SequenceATCGTCTGTTGCGCG
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol