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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD18 (TS1/18)

CD18 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Conjugate

5CFLX

Barcode Sequence

CGTGGTTGGAAGCAC

CloneNo.

TS1/18

Cat no : G65190-1-5C

Synonyms

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7

Validation Data Gallery

Filter:
  • Single Cell Sequencing experiment G65190-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65190-1-5C on Resting PBMC

    G65190-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65190-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65190-1-5C on PBMC treated with 4hr LPS + TI

    G65190-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65190-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65190-1-5C on Resting PBMC

    G65190-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65190-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65190-1-5C on PBMC treated with 4hr LPS + TI

    G65190-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65190-1-5C targets CD18 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Immunogen Human Beta 2 Integrin 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD18 (TS1/18)
Calculated molecular weight 85 kDa
GenBank accession numberBC005861
Gene symbol CD18
Gene ID (NCBI) 3689
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCGTGGTTGGAAGCACCCCATATAAGAAA
Barcode SequenceCGTGGTTGGAAGCAC
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol