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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiProTM 5CFLX Anti-Human CD3 (OKT3)

CD3 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

TCGGATAAGACCTTC

CloneNo.

OKT3

Cat no : G65133-1-5C

Synonyms

CD3, CD3E, T3E, TCRE

Validation Data Gallery

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  • Single Cell Sequencing experiment G65133-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65133-1-5C on Resting PBMC

    G65133-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65133-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65133-1-5C on PBMC treated with 4hr LPS + TI

    G65133-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65133-1-5C targets CD3 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Oligo Conjugate
Type Monoclonal
Immunogen Human T cells 相同性解析による交差性が予測される生物種
Full Name MultiProTM 5CFLX Anti-Human CD3 (OKT3)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene symbol CD3E
Gene ID (NCBI) 916
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTCGGATAAGACCTTCCCCATATAAGAAA
Barcode SequenceTCGGATAAGACCTTC
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol