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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD4 (RPA-T4)

CD4 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1

Reactivity

Human

Conjugate

5CFLX

Barcode Sequence

GTCTTACAACGCAGT

CloneNo.

RPA-T4

Cat no : G65143-1-5C

Synonyms

CD4, CD4 molecule, CD4mut

Validation Data Gallery

Filter:
  • Single Cell Sequencing experiment G65143-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65143-1-5C on Resting PBMC

    G65143-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65143-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65143-1-5C on PBMC treated with 4hr LPS + TI

    G65143-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65143-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65143-1-5C on Resting PBMC

    G65143-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65143-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65143-1-5C on PBMC treated with 4hr LPS + TI

    G65143-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65143-1-5C targets CD4 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD4 (RPA-T4)
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGTCTTACAACGCAGTCCCATATAAGAAA
Barcode SequenceGTCTTACAACGCAGT
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol