Validation Data Gallery
Tested Applications
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Recommended dilution
| Application | Dilution |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
G66224-1-5C targets CD43 in Single Cell (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Oligo Conjugate |
| Type | Monoclonal |
| Immunogen | CD43 fusion protein Ag23595 相同性解析による交差性が予測される生物種 |
| Full Name | MultiPro® 5CFLX Anti-Human CD43 (2A11D6) |
| Calculated molecular weight | 400 aa, 43 kDa |
| GenBank accession number | BC012350 |
| Gene Symbol | CD43 |
| Gene ID (NCBI) | 6693 |
| ENSEMBL Gene ID | ENSG00000197471 |
| RRID | AB_3673945 |
| Conjugate | 5CFLX |
| Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGCCACAATCCTAACGGCCCATATAAGAAA |
| Barcode Sequence | CCACAATCCTAACGG |
| Form | Liquid |
| UNIPROT ID | P16150 |
| Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
| Storage Conditions | 2-8°C Stable for one year after shipment. |
Background Information
CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.
Protocols
| MultiPro™ Cell Surface and Intracellular Staining Protocol | Download protocol |
| 10x Genomics Cell Surface Protein Only Staining Protocol | Download protocol |