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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiProTM 5CFLX Anti-Human CD43 (2A11D6)

CD43 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

CCACAATCCTAACGG

CloneNo.

2A11D6

Cat no : G66224-1-5C

Synonyms

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN

Validation Data Gallery

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  • Single Cell Sequencing experiment G66224-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66224-1-5C on Resting PBMC

    G66224-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66224-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66224-1-5C on PBMC treated with 4hr LPS + TI

    G66224-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G66224-1-5C targets CD43 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Oligo Conjugate
Type Monoclonal
Immunogen CD43 fusion protein Ag23595 相同性解析による交差性が予測される生物種
Full Name MultiProTM 5CFLX Anti-Human CD43 (2A11D6)
Calculated molecular weight 400 aa, 43 kDa
GenBank accession numberBC012350
Gene symbol SPN
Gene ID (NCBI) 6693
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCCACAATCCTAACGGCCCATATAAGAAA
Barcode SequenceCCACAATCCTAACGG
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol