MultiPro® 5CFLX Anti-Human CD43 (2A11D6)
CD43 Monoclonal Antibody for Single Cell (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
Conjugate
5CFLX
Barcode Sequence
CCACAATCCTAACGG
CloneNo.
2A11D6
Cat no : G66224-1-5C
Synonyms
Validation Data Gallery
Tested Applications
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product |
Recommended dilution
Application | Dilution |
---|---|
SINGLE CELL (INTRA) | <0.5ug/test |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
G66224-1-5C targets CD43 in Single Cell (Intra) applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Oligo Conjugate |
Type | Monoclonal |
Immunogen | CD43 fusion protein Ag23595 相同性解析による交差性が予測される生物種 |
Full Name | MultiPro® 5CFLX Anti-Human CD43 (2A11D6) |
Calculated molecular weight | 400 aa, 43 kDa |
GenBank accession number | BC012350 |
Gene symbol | CD43 |
Gene ID (NCBI) | 6693 |
Conjugate | 5CFLX |
Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGCCACAATCCTAACGGCCCATATAAGAAA |
Barcode Sequence | CCACAATCCTAACGG |
Form | Liquid |
Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
Storage Conditions | 2-8°C Stable for one year after shipment. |
Background Information
CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.
Protocols
MultiPro™ Cell Surface and Intracellular Staining Protocol | Download protocol |
10x Genomics Cell Surface Protein Only Staining Protocol | Download protocol |