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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Conjugate

5CFLX

Barcode Sequence

TTACTCCTCCAGGTT

CloneNo.

F8-11-13

Cat no : G65226-1-5C

Synonyms

B220, CD45, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

Validation Data Gallery

Filter:
  • Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    G65226-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    G65226-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    G65226-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    G65226-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65226-1-5C targets CD45RA in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Immunogen Human T lymphocytes 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD45RA (F8-11-13)
Calculated molecular weight 1304 aa, 147 kDa
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTTACTCCTCCAGGTTCCCATATAAGAAA
Barcode SequenceTTACTCCTCCAGGTT
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol