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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD5 (UCHT2)

CD5 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Conjugate

5CFLX

Barcode Sequence

GTGCCTCGCATTGCA

CloneNo.

UCHT2

Cat no : G65152-1-5C

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

Validation Data Gallery

Filter:
  • Single Cell Sequencing experiment G65152-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65152-1-5C on Resting PBMC

    G65152-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65152-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65152-1-5C on PBMC treated with 4hr LPS + TI

    G65152-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65152-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65152-1-5C on Resting PBMC

    G65152-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65152-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65152-1-5C on PBMC treated with 4hr LPS + TI

    G65152-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65152-1-5C targets CD5 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD5 (UCHT2)
Calculated molecular weight 495 aa, 55 kDa
GenBank accession numberBC027901
Gene symbol CD5
Gene ID (NCBI) 921
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGTGCCTCGCATTGCACCCATATAAGAAA
Barcode SequenceGTGCCTCGCATTGCA
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol