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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD62L (DREG56)

CD62L Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / Mouse IgG1

Reactivity

Human

Conjugate

5CFLX

Barcode Sequence

TTCCGGTTCTTGAAT

CloneNo.

DREG56

Cat no : G65167-1-5C

Synonyms

CD62L, gp90 MEL, hLHRc, L selectin, LAM 1, LAM1, LECAM1, Leu 8, Leukocyte adhesion molecule 1, LNHR, LSEL, Lyam 1, LYAM1, Lymph node homing receptor, PLNHR, selectin L, SELL, TQ1

Validation Data Gallery

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  • Single Cell Sequencing experiment G65167-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65167-1-5C on Resting PBMC

    G65167-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65167-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65167-1-5C on Resting PBMC

    G65167-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65167-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65167-1-5C on PBMC treated with 4hr LPS + TI

    G65167-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65167-1-5C targets CD62L in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1
Class Oligo Conjugate
Type Monoclonal
Immunogen PMA-activated human peripheral blood leukocytes 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD62L (DREG56)
Calculated molecular weight 42 kDa
GenBank accession numberBC020758
Gene symbol CD62L
Gene ID (NCBI) 6402
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTTCCGGTTCTTGAATCCCATATAAGAAA
Barcode SequenceTTCCGGTTCTTGAAT
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol