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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD64 (10.1)

CD64 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Conjugate

5CFLX

Barcode Sequence

CCATCGCCAGCGCGG

CloneNo.

10.1

Cat no : G65253-1-5C

Synonyms

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I

Validation Data Gallery

Filter:
  • Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    G65253-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    G65253-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    G65253-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    G65253-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65253-1-5C targets CD64 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Immunogen Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD64 (10.1)
Calculated molecular weight 374 aa, 43 kDa
GenBank accession numberBC032634
Gene symbol FCGR1A
Gene ID (NCBI) 2209
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCCATCGCCAGCGCGGCCCATATAAGAAA
Barcode SequenceCCATCGCCAGCGCGG
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol