MultiProTM-5CFLX Anti-Human CD8 (SK1)
CD8 Monoclonal Antibody for
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
Conjugate
5CFLX Fluorescent Dye
CloneNo.
SK1
Cat no : G65146-1-5C
Synonyms
Validation Data Gallery
Tested Applications
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product |
Recommended dilution
Application | Dilution |
---|---|
SINGLE CELL (INTRA) | SINGLE CELL (INTRA) : <0.5ug/test |
Sample-dependent, check data in validation data gallery |
Product Information
G65146-1-5C targets CD8 in applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | N/A 相同性解析による交差性が予測される生物種 |
Full Name | CD8a molecule |
Calculated molecular weight | 235 aa, 26 kDa |
GenBank accession number | BC025715 |
Gene symbol | CD8A |
Gene ID (NCBI) | 925 |
Conjugate | 5CFLX Fluorescent Dye |
Form | Liquid |
Purification Method | |
Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
Storage Conditions | 2-8°C |
Background Information
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).