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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD8 (UCHT4)

CD8 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Conjugate

5CFLX

Barcode Sequence

TGGAATCTAATACCA

CloneNo.

UCHT4

Cat no : G65204-1-5C

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

Validation Data Gallery

Filter:
  • Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    G65204-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    G65204-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    G65204-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    G65204-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65204-1-5C targets CD8 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Oligo Conjugate
Type Monoclonal
Immunogen Thymocytes and Sézary T cells 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD8 (UCHT4)
Calculated molecular weight 235 aa, 26 kDa
GenBank accession numberBC025715
Gene symbol CD8A
Gene ID (NCBI) 925
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTGGAATCTAATACCACCCATATAAGAAA
Barcode SequenceTGGAATCTAATACCA
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol