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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human CD81 (5A6)

CD81 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

GTCAATAGTTGGCTA

CloneNo.

5A6

Cat no : G65195-1-5C

Synonyms

CD81, CD81 antigen, CD81 molecule, Cell and organelle markers, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

Validation Data Gallery

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  • Single Cell Sequencing experiment G65195-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65195-1-5C on Resting PBMC

    G65195-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65195-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65195-1-5C on PBMC treated with 4hr LPS + TI

    G65195-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65195-1-5C targets CD81 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Immunogen Human OCI-LY8 cell line 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD81 (5A6)
Calculated molecular weight 26 kDa
GenBank accession numberBC002978
Gene symbol CD81
Gene ID (NCBI) 975
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGTCAATAGTTGGCTACCCATATAAGAAA
Barcode SequenceGTCAATAGTTGGCTA
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol