Validation Data Gallery
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Tested Applications
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Recommended dilution
| Application | Dilution |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
G66321-1-5C targets Fascin in Single Cell (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Oligo Conjugate |
| Type | Monoclonal |
| Immunogen | Fascin fusion protein Ag6074 相同性解析による交差性が予測される生物種 |
| Full Name | MultiPro® 5CFLX Anti-Human Fascin (1B5G3) |
| Calculated molecular weight | 55 kDa |
| GenBank accession number | BC000521 |
| Gene Symbol | Fascin |
| Gene ID (NCBI) | 6624 |
| ENSEMBL Gene ID | ENSG00000075618 |
| RRID | AB_3673947 |
| Conjugate | 5CFLX |
| Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGGCCGTTCGCGGTACGCCCATATAAGAAA |
| Barcode Sequence | GCCGTTCGCGGTACG |
| Form | Liquid |
| UNIPROT ID | Q16658 |
| Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
| Storage Conditions | 2-8°C Stable for one year after shipment. |
Background Information
Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.
Protocols
| MultiPro™ Cell Surface and Intracellular Staining Protocol | Download protocol |
| 10x Genomics Cell Surface Protein Only Staining Protocol | Download protocol |