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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human Fascin (1B5G3)

Fascin Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

GCCGTTCGCGGTACG

CloneNo.

1B5G3

Cat no : G66321-1-5C

Synonyms

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FSCN1, HSN, p55, Singed like protein, SNL

Validation Data Gallery

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  • Single Cell Sequencing experiment G66321-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66321-1-5C on Resting PBMC

    G66321-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66321-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66321-1-5C on PBMC treated with 4hr LPS + TI

    G66321-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G66321-1-5C targets Fascin in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Oligo Conjugate
Type Monoclonal
Immunogen Fascin fusion protein Ag6074 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human Fascin (1B5G3)
Calculated molecular weight 55 kDa
GenBank accession numberBC000521
Gene symbol Fascin
Gene ID (NCBI) 6624
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGCCGTTCGCGGTACGCCCATATAAGAAA
Barcode SequenceGCCGTTCGCGGTACG
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol