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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human IL-1 Beta (2A1B4)

IL-1 Beta Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

TGAGTGGATTGTTCT

CloneNo.

2A1B4

Cat no : G66737-1-5C

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Validation Data Gallery

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  • Single Cell Sequencing experiment G66737-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66737-1-5C on Resting PBMC

    G66737-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66737-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66737-1-5C on PBMC treated with 4hr LPS + TI

    G66737-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G66737-1-5C targets IL-1 Beta in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Immunogen IL-1 Beta fusion protein Ag26030 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human IL-1 Beta (2A1B4)
Calculated molecular weight 269 aa, 31 kDa
GenBank accession numberBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTGAGTGGATTGTTCTCCCATATAAGAAA
Barcode SequenceTGAGTGGATTGTTCT
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol