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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human L-selectin/CD62L (Polyclonal)

L-selectin / CD62L Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

AGCTTCGCTGAACCG

Cat no : G26477-1-5C

Synonyms

CD62L, gp90 MEL, hLHRc, LAM 1, LAM1, LECAM1, Leu 8, Leukocyte adhesion molecule 1, LNHR, LSEL, L-selectin, Lyam 1, LYAM1, Lymph node homing receptor, PLNHR, selectin L, SELL, TQ1

Validation Data Gallery

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  • Single Cell Sequencing experiment G26477-1-5C on Resting PBMC

    Single Cell Sequencing experiment G26477-1-5C on Resting PBMC

    G26477-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G26477-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G26477-1-5C on PBMC treated with 4hr LPS + TI

    G26477-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G26477-1-5C targets L-selectin / CD62L in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Immunogen L-selectin / CD62L fusion protein Ag24063 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human L-selectin/CD62L (Polyclonal)
Calculated molecular weight 42 kDa
GenBank accession numberBC020758
Gene symbol CD62L
Gene ID (NCBI) 6402
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGAGCTTCGCTGAACCGCCCATATAAGAAA
Barcode SequenceAGCTTCGCTGAACCG
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol