Validation Data Gallery
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Tested Applications
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Recommended dilution
| Application | Dilution |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
G81004-1-5C targets LC3 in Single Cell (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Oligo Conjugate |
| Type | Recombinant |
| Immunogen | LC3 fusion protein Ag6144 相同性解析による交差性が予測される生物種 |
| Full Name | MultiPro® 5CFLX Anti-Human LC3 (5P12) |
| Calculated molecular weight | 15 kDa |
| GenBank accession number | BC067797 |
| Gene Symbol | LC3B |
| Gene ID (NCBI) | 81631 |
| ENSEMBL Gene ID | ENSG00000140941 |
| RRID | AB_3673976 |
| Conjugate | 5CFLX |
| Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGCTTGACAATCGTGAACCCATATAAGAAA |
| Barcode Sequence | CTTGACAATCGTGAA |
| Form | Liquid |
| UNIPROT ID | Q9GZQ8 |
| Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
| Storage Conditions | 2-8°C Stable for one year after shipment. |
Background Information
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane.
Protocols
| MultiPro™ Cell Surface and Intracellular Staining Protocol | Download protocol |
| 10x Genomics Cell Surface Protein Only Staining Protocol | Download protocol |