Validation Data Gallery
Tested Applications
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Recommended dilution
| Application | Dilution |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
G20189-1-5C targets MAFB in Single Cell (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Oligo Conjugate |
| Type | Polyclonal |
| Immunogen | MAFB fusion protein Ag14102 相同性解析による交差性が予測される生物種 |
| Full Name | MultiPro® 5CFLX Anti-Human MAFB (Polyclonal) |
| Calculated molecular weight | 323 aa, 36 kDa |
| GenBank accession number | BC036689 |
| Gene Symbol | MAFB |
| Gene ID (NCBI) | 9935 |
| ENSEMBL Gene ID | ENSG00000204103 |
| RRID | AB_3673893 |
| Conjugate | 5CFLX |
| Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGTCGCGGACCAGGAATCCCATATAAGAAA |
| Barcode Sequence | TCGCGGACCAGGAAT |
| Form | Liquid |
| UNIPROT ID | Q9Y5Q3 |
| Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
| Storage Conditions | 2-8°C Stable for one year after shipment. |
Background Information
MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).
Protocols
| MultiPro™ Cell Surface and Intracellular Staining Protocol | Download protocol |
| 10x Genomics Cell Surface Protein Only Staining Protocol | Download protocol |