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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human MAFB (Polyclonal)

MAFB Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

TCGCGGACCAGGAAT

Cat no : G20189-1-5C

Synonyms

KRML, Maf B, MAFB, Transcription factor MafB

Validation Data Gallery

Filter:
  • Single Cell Sequencing experiment G20189-1-5C on Resting PBMC

    Single Cell Sequencing experiment G20189-1-5C on Resting PBMC

    G20189-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G20189-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G20189-1-5C on PBMC treated with 4hr LPS + TI

    G20189-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G20189-1-5C targets MAFB in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Immunogen MAFB fusion protein Ag14102 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human MAFB (Polyclonal)
Calculated molecular weight 323 aa, 36 kDa
GenBank accession numberBC036689
Gene symbol MAFB
Gene ID (NCBI) 9935
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTCGCGGACCAGGAATCCCATATAAGAAA
Barcode SequenceTCGCGGACCAGGAAT
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol