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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human MUM1/IRF4 (Polyclonal)

MUM1/IRF4 Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

AGTGCTTCTAGCCGA

Cat no : G11247-2-5C

Synonyms

IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, MUM1/IRF4, NF EM5

Validation Data Gallery

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  • Single Cell Sequencing experiment G11247-2-5C on Resting PBMC

    Single Cell Sequencing experiment G11247-2-5C on Resting PBMC

    G11247-2-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G11247-2-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G11247-2-5C on PBMC treated with 4hr LPS + TI

    G11247-2-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G11247-2-5C targets MUM1/IRF4 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Immunogen MUM1/IRF4 fusion protein Ag1762 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human MUM1/IRF4 (Polyclonal)
Calculated molecular weight 52 kDa
GenBank accession numberBC015752
Gene symbol IRF4
Gene ID (NCBI) 3662
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGAGTGCTTCTAGCCGACCCATATAAGAAA
Barcode SequenceAGTGCTTCTAGCCGA
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol