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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiProTM-5CFLX Anti-Human NFKB2 (6A10E9)

NFKB2 Monoclonal Antibody for

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Conjugate

5CFLX Fluorescent Dye

CloneNo.

6A10E9

Cat no : G66920-1-5C

Synonyms

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, Oncogene Lyt 10, p100

Validation Data Gallery

Applications:
  • Single Cell Sequencing experiment G66920-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66920-1-5C on Resting PBMC

    G66920-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66920-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66920-1-5C on PBMC treated with 4hr LPS + TI

    G66920-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)SINGLE CELL (INTRA) : <0.5ug/test
Sample-dependent, check data in validation data gallery

Product Information

G66920-1-5C targets NFKB2 in applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFKB2 fusion protein Ag28543 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Calculated molecular weight 97 kDa
GenBank accession numberBC002844
Gene symbol NFKB2
Gene ID (NCBI) 4791
Conjugate 5CFLX Fluorescent Dye
Form Liquid
Purification Method
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.