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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human SP1 (4G3H3)

SP1 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

TCATTGCGGATTAGC

CloneNo.

4G3H3

Cat no : G66508-1-5C

Synonyms

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1

Validation Data Gallery

Filter:
  • Single Cell Sequencing experiment G66508-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66508-1-5C on Resting PBMC

    G66508-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66508-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66508-1-5C on PBMC treated with 4hr LPS + TI

    G66508-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G66508-1-5C targets SP1 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Immunogen SP1 fusion protein Ag16720 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human SP1 (4G3H3)
Calculated molecular weight 785 aa, 81 kDa
GenBank accession numberBC062539
Gene symbol SP1
Gene ID (NCBI) 6667
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTCATTGCGGATTAGCCCCATATAAGAAA
Barcode SequenceTCATTGCGGATTAGC
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol