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Validation Data Gallery

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  • Single Cell Sequencing experiment G66618-2-5C on Resting PBMC

    Single Cell Sequencing experiment G66618-2-5C on Resting PBMC

    G66618-2-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66618-2-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66618-2-5C on PBMC treated with 4hr LPS + TI

    G66618-2-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MultiPro® 5CFLX Anti-Human SPI1 (2H3D3)

SPI1 Monoclonal Antibody for Single Cell (Intra)
Cat No. G66618-2-5C
Clone No.2H3D3

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Single Cell (Intra)

Synonyms

31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G66618-2-5C targets SPI1 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human SPI1 (2H3D3)
Calculated molecular weight 31 kDa
GenBank accession numberNM_003120
Gene Symbol SPI1
Gene ID (NCBI) 6688
ENSEMBL Gene IDENSG00000066336
RRIDAB_3673957
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGCAATAACCTCCGCGCCCATATAAGAAA
Barcode SequenceGCAATAACCTCCGCG
Form Liquid
UNIPROT IDP17947
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol