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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human TBX21/T-bet (Polyclonal)

TBX21,T-bet Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

GGTTCAATCCGGACG

Cat no : G13700-1-5C

Synonyms

T bet, T box 21, T box protein 21, T PET, TBET, TBLYM, TBX21, TBX21,T bet, TBX21,T-bet, Transcription factor TBLYM

Validation Data Gallery

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  • Single Cell Sequencing experiment G13700-1-5C on Resting PBMC

    Single Cell Sequencing experiment G13700-1-5C on Resting PBMC

    G13700-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G13700-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G13700-1-5C on PBMC treated with 4hr LPS + TI

    G13700-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G13700-1-5C targets TBX21,T-bet in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Immunogen TBX21,T-bet fusion protein Ag4618 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human TBX21/T-bet (Polyclonal)
Calculated molecular weight 535 aa, 58 kDa
GenBank accession numberBC039739
Gene symbol TBX21/T-bet
Gene ID (NCBI) 30009
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGGTTCAATCCGGACGCCCATATAAGAAA
Barcode SequenceGGTTCAATCCGGACG
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol