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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human TCF7 (Polyclonal)

TCF7 Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

CGCCTATAGAGACGC

Cat no : G14464-1-5C

Synonyms

T cell factor 1, TCF 1, TCF 7, TCF1, TCF7, Transcription factor 7

Validation Data Gallery

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  • Single Cell Sequencing experiment G14464-1-5C on Resting PBMC

    Single Cell Sequencing experiment G14464-1-5C on Resting PBMC

    G14464-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G14464-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G14464-1-5C on PBMC treated with 4hr LPS + TI

    G14464-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G14464-1-5C targets TCF7 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Immunogen TCF7 fusion protein Ag5792 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human TCF7 (Polyclonal)
Calculated molecular weight 42 kDa
GenBank accession numberBC048769
Gene symbol TCF1/TCF7
Gene ID (NCBI) 6932
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCGCCTATAGAGACGCCCCATATAAGAAA
Barcode SequenceCGCCTATAGAGACGC
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

TCF7, also known as TCF1, belongs to the TCF/LEF family. TCF7 is part of the Wnt signaling pathway and plays an important role in the differentiation and self-renewal of memory T cells. It has been found that TCF7 is necessary to revive T cells in response to PD-1 blockade against viral infection or cancer. TCF7 is mainly expressed in T-cells and is also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. TCF7 has 16 isoforms with the molecular mass of 28-54 kDa (PMID: 34044317).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol