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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human TGFBI/BIGH3 (3E11D11)

TGFBI / BIGH3 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

TCCAAGGTAACTGCG

CloneNo.

3E11D11

Cat no : G60007-1-5C

Synonyms

Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3

Validation Data Gallery

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  • Single Cell Sequencing experiment G60007-1-5C on Resting PBMC

    Single Cell Sequencing experiment G60007-1-5C on Resting PBMC

    G60007-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G60007-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G60007-1-5C on PBMC treated with 4hr LPS + TI

    G60007-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G60007-1-5C targets TGFBI / BIGH3 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Oligo Conjugate
Type Monoclonal
Immunogen TGFBI / BIGH3 fusion protein Ag0241 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human TGFBI/BIGH3 (3E11D11)
Calculated molecular weight 683 aa, 75 kDa
GenBank accession numberBC000097
Gene symbol TGFBI
Gene ID (NCBI) 7045
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTCCAAGGTAACTGCGCCCATATAAGAAA
Barcode SequenceTCCAAGGTAACTGCG
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol