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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiProTM 5CFLX Anti-Human Vimentin (3H9D1)

Vimentin Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

ACATGCCTAGCTCCG

CloneNo.

3H9D1

Cat no : G60330-1-5C

Synonyms

VIM, Vimentin

Validation Data Gallery

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  • Single Cell Sequencing experiment G60330-1-5C on Resting PBMC

    Single Cell Sequencing experiment G60330-1-5C on Resting PBMC

    G60330-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G60330-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G60330-1-5C on PBMC treated with 4hr LPS + TI

    G60330-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G60330-1-5C targets Vimentin in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Immunogen Vimentin fusion protein Ag0489 相同性解析による交差性が予測される生物種
Full Name MultiProTM 5CFLX Anti-Human Vimentin (3H9D1)
Calculated molecular weight 466 aa, 54 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGACATGCCTAGCTCCGCCCATATAAGAAA
Barcode SequenceACATGCCTAGCTCCG
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol