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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiPro® 5CFLX Anti-Human ZAP70 (1A11G9)

ZAP70 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

CTCTACCGCGCGACG

CloneNo.

1A11G9

Cat no : G60200-1-5C

Synonyms

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

Validation Data Gallery

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  • Single Cell Sequencing experiment G60200-1-5C on Resting PBMC

    Single Cell Sequencing experiment G60200-1-5C on Resting PBMC

    G60200-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G60200-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G60200-1-5C on PBMC treated with 4hr LPS + TI

    G60200-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of G60200-1-5C is ZAP70 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Oligo Conjugate
Type Monoclonal
Immunogen ZAP70 fusion protein Ag7943 相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human ZAP70 (1A11G9)
Calculated molecular weight 70 kDa
GenBank accession numberBC039039
Gene symbol ZAP70
Gene ID (NCBI) 7535
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCTCTACCGCGCGACGCCCATATAAGAAA
Barcode SequenceCTCTACCGCGCGACG
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol