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Validation Data Gallery

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  • Western Blot (WB) analysis of pig heart tissue using Myocilin Monoclonal antibody (60357-1-Ig)

    WB analysis of pig heart using 60357-1-Ig (same clone as 60357-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using Myocilin Monoclonal antibody (60357-1-Ig)

    WB analysis of rat heart using 60357-1-Ig (same clone as 60357-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using Myocilin Monoclonal antibody (60357-1-Ig)

    WB analysis of human skeletal muscle using 60357-1-Ig (same clone as 60357-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using Myocilin Monoclonal antibody (60357-1-Ig)

    WB analysis of human spleen using 60357-1-Ig (same clone as 60357-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Myocilin Monoclonal antibody (60357-1-Ig)

    IHC staining of human heart using 60357-1-Ig (same clone as 60357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Myocilin Monoclonal antibody (60357-1-Ig)

    IHC staining of human heart using 60357-1-Ig (same clone as 60357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Myocilin Monoclonal antibody (60357-1-Ig)

    IHC staining of human skeletal muscle using 60357-1-Ig (same clone as 60357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Myocilin Monoclonal antibody (60357-1-Ig)

    IHC staining of human skeletal muscle using 60357-1-Ig (same clone as 60357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

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Myocilin Monoclonal antibody, PBS Only

Myocilin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60357-1-PBS
Clone No.4B1F6

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

GLC1A, GPOA, JOAG, JOAG1, MYOC, myocilin, TIGR

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60357-1-PBS targets Myocilin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Myocilin fusion protein Ag5492 相同性解析による交差性が予測される生物種
Full Name myocilin, trabecular meshwork inducible glucocorticoid response
Calculated molecular weight 57 kDa
Observed molecular weight 50-57 kDa
GenBank accession numberBC029261
Gene Symbol Myocilin
Gene ID (NCBI) 4653
RRIDAB_2881465
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99972
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622).