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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Myogenin Monoclonal antibody, PBS Only (Capture)

Myogenin Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C10B9

Cat no : 67082-1-PBS

Synonyms

MYOG, Myf-4, MYF4, Myf 4, Class C basic helix-loop-helix protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using Myogenin Monoclonal antibody (67082-1-Ig)

    WB analysis of human skeletal muscle using 67082-1-Ig (same clone as 67082-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Myogenin Monoclonal antibody (67082-1-Ig)

    WB analysis of human heart using 67082-1-Ig (same clone as 67082-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using Myogenin Monoclonal antibody (67082-1-Ig)

    WB analysis of pig heart using 67082-1-Ig (same clone as 67082-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using Myogenin Monoclonal antibody (67082-1-Ig)

    WB analysis of rat heart using 67082-1-Ig (same clone as 67082-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67082-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51032-2

    Cytometric bead array standard curve of MP51032-2

    Cytometric bead array standard curve of MP51032-2, Myogenin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67082-1-PBS. Detection antibody: 67082-3-PBS. Standard:Ag6414. Range: 1.563-25 ng/mL.

  • Cytometric bead array standard curve of MP51032-3

    Cytometric bead array standard curve of MP51032-3

    Cytometric bead array standard curve of MP51032-3, Myogenin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67082-1-PBS. Detection antibody: 67082-4-PBS. Standard:Ag6414. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67082-1-PBS targets Myogenin as part of a matched antibody pair:

MP51032-2: 67082-1-PBS capture and 67082-3-PBS detection (validated in Cytometric bead array)

MP51032-3: 67082-1-PBS capture and 67082-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Myogenin fusion protein Ag6414 相同性解析による交差性が予測される生物種
Full Name myogenin (myogenic factor 4)
Calculated molecular weight 25 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC053899
Gene symbol Myogenin
Gene ID (NCBI) 4656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYOG (encoded Myogenin protein) is a muscle-specific transcription factor which belongs to a family of basic helix-loop-helix transcription factors. It plays a role in muscle differentiation, cell cycle exit and muscle atrophy(PMID: 30315160). It can be initiate the myogenic program in myoblasts, fiblasts and veritey of other cell types. Diseases associated with MYOG include Embryonal Rhabdomyosarcoma and Gallbladder Sarcoma.