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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Myosin Light Chain 2/MLC-2V Monoclonal antibody, PBS Only

Myosin Light Chain 2/MLC-2V Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A1F1

Cat no : 60229-1-PBS

Synonyms

CMH10, MLC 2, MLC 2v, MLC2, MLC2v, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Myosin Light Chain 2/MLC-2V Monoclonal antibody (60229-1-Ig)

    WB analysis using 60229-1-Ig (same clone as 60229-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using Myosin Light Chain 2/MLC-2V Monoclonal antibody (60229-1-Ig)

    WB analysis of pig heart using 60229-1-Ig (same clone as 60229-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using Myosin Light Chain 2/MLC-2V Monoclonal antibody (60229-1-Ig)

    WB analysis of rat heart using 60229-1-Ig (same clone as 60229-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Myosin Light Chain 2/MLC-2V Monoclonal antibody (60229-1-Ig)

    IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Myosin Light Chain 2/MLC-2V Monoclonal antibody (60229-1-Ig)

    IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Myosin Light Chain 2/MLC-2V Monoclonal antibody (60229-1-Ig)

    IHC staining of mouse skeletal muscle using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Myosin Light Chain 2/MLC-2V Monoclonal antibody (60229-1-Ig)

    IHC staining of mouse heart using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Myosin Light Chain 2/MLC-2V Monoclonal antibody (60229-1-Ig)

    IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cell using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Myosin Light Chain 2/MLC-2V Monoclonal antibody (60229-1-Ig)

    IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Myosin Light Chain 2/MLC-2V antibody (60229-1-Ig, Clone: 5A1F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60229-1-PBS targets Myosin Light Chain 2/MLC-2V in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Myosin Light Chain 2/MLC-2V fusion protein Ag1356 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 2, regulatory, cardiac, slow
Calculated molecular weight 19 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC015821
Gene symbol MYL2
Gene ID (NCBI) 4633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.