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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

N-cadherin Polyclonal antibody

N-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30803-1-AP

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using N-cadherin Polyclonal antibody (30803-1-AP)

    WB analysis using 30803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30803-1-AP (N-cadherin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using N-cadherin Polyclonal antibody (30803-1-AP)

    IHC staining of mouse heart using 30803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30803-1-AP (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using N-cadherin Polyclonal antibody (30803-1-AP)

    IHC staining of mouse heart using 30803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30803-1-AP (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using N-cadherin Polyclonal antibody (30803-1-AP)

    IF Staining of C2C12 using 30803-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using N-cadherin antibody (30803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, rat brain tissue, PC-3 cells, C2C12 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30803-1-AP targets N-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight100 kDa
Observed molecular weight130 kDa
GenBank accession numberNM_001792.4
Gene symbol N-cadherin
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).

Protocols

Product Specific Protocols
WB protocol for N-cadherin antibody 30803-1-APDownload protocol
IHC protocol for N-cadherin antibody 30803-1-APDownload protocol
IF protocol for N-cadherin antibody 30803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols