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  • Featured Product
  • KD/KO Validated

N-cadherin Monoclonal antibody, PBS Only (Capture)

N-cadherin Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D8B3

Cat no : 66219-1-PBS

Synonyms

CDHN, CDH2, CD325, Cadherin-2, Cadherin 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis of HEK-293 using 66219-1-Ig (same clone as 66219-1-PBS)

    WB result of N-cadherin antibody (66219-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis using 66219-1-Ig (same clone as 66219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis of PC-3 using 66219-1-Ig (same clone as 66219-1-PBS)

    Untreated and PNGase F-treated lysates of PC-3 cells were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MKN-45 cells using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis of MKN-45 using 66219-1-Ig (same clone as 66219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis of human heart using 66219-1-Ig (same clone as 66219-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis of pig brain using 66219-1-Ig (same clone as 66219-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis using 66219-1-Ig (same clone as 66219-1-PBS)

    HEK-293 (N-cadherin+), PC-3 cells (N-cadherin+), C6 cells (N-cadherin+), NIH/3T3 cells (N-cadherin+), MCF-7 (N-cadherin-), A431 (N-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of human malignant melanoma using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of human malignant melanoma using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of mouse brain using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of mouse brain using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of rat heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of rat heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and CoraLite594-Conjugated Goat Anti-Mouse IgG(H+L). The section was co-stained with 11313-2-AP (alpha Actinin) in green. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50439-2

    Cytometric bead array standard curve of MP50439-2

    Cytometric bead array standard curve of MP50439-2, N-cadherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66219-1-PBS. Detection antibody: 66219-2-PBS. Standard:Eg0701. Range: 0.781-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66219-1-PBS targets N-cadherin as part of a matched antibody pair:

MP50439-2: 66219-1-PBS capture and 66219-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N-cadherin fusion protein Ag4996 相同性解析による交差性が予測される生物種
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC036470
Gene symbol N-cadherin
Gene ID (NCBI) 1000
RRIDAB_2881610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).