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  • Featured Product
  • KD/KO Validated

N-cadherin Monoclonal antibody, PBS Only

N-cadherin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat, rabbit

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D8B3

Cat no : 66219-1-PBS

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, N-cadherin, Neural cadherin

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis of HEK-293 using 66219-1-Ig (same clone as 66219-1-PBS)

    WB result of N-cadherin antibody (66219-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis using 66219-1-Ig (same clone as 66219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis of PC-3 using 66219-1-Ig (same clone as 66219-1-PBS)

    Untreated and PNGase F-treated lysates of PC-3 cells were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MKN-45 cells using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis of MKN-45 using 66219-1-Ig (same clone as 66219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis using 66219-1-Ig (same clone as 66219-1-PBS)

    HEK-293 (N-cadherin+), PC-3 cells (N-cadherin+), C6 cells (N-cadherin+), NIH/3T3 cells (N-cadherin+), MCF-7 (N-cadherin-), A431 (N-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis of human heart using 66219-1-Ig (same clone as 66219-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    WB analysis of pig brain using 66219-1-Ig (same clone as 66219-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of human malignant melanoma using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of human malignant melanoma using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of mouse brain using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of mouse brain using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of rat heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IHC staining of rat heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 11313-2-AP (alpha Actinin) in green. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using N-cadherin Monoclonal antibody (66219-1-Ig)

    IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66219-1-PBS targets N-cadherin in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, mouse, rat, rabbit samples.

Tested Reactivity human, pig, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N-cadherin fusion protein Ag4996 相同性解析による交差性が予測される生物種
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC036470
Gene symbol CDH2
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).