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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

N-cadherin Recombinant antibody, PBS Only

N-cadherin Recombinant Antibody for WB, IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230259H5

Cat no : 82968-1-PBS

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using N-cadherin Recombinant antibody (82968-1-RR)

    WB analysis using 82968-1-RR (same clone as 82968-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82968-1-RR (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using N-cadherin Recombinant antibody (82968-1-RR)

    IF Staining of HeLa using 82968-1-RR (same clone as 82968-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using N-cadherin Recombinant antibody (82968-1-RR)

    FC experiment of HeLa using 82968-1-RR (same clone as 82968-1-PBS)

    1x10^6 HeLa cells were surface stained with 0.25 ug N-cadherin Recombinant antibody (82968-1-RR, Clone: 230259H5) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82968-1-PBS targets N-cadherin in WB, IF, FC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight100 kDa
Observed molecular weight130 kDa
GenBank accession numberNM_001792.4
Gene symbol CDH2
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also includes E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).