Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23892-1-AP targets N4BP2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N4BP2 fusion protein Ag20943 相同性解析による交差性が予測される生物種 |
| Full Name | NEDD4 binding protein 2 |
| Calculated molecular weight | 1770 aa, 199 kDa |
| Observed molecular weight | 199 kDa |
| GenBank accession number | BC126466 |
| Gene Symbol | N4BP2 |
| Gene ID (NCBI) | 55728 |
| RRID | AB_3669422 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q86UW6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
N4BP2 (Nedd4 binding protein 2) is a Bcl-3 binding protein, which contains a polynucleotide kinase domain (PNK) at the N-terminus and a Small MutS Related (Smr) domain with nicking endonuclease activity near C-terminus. N4BP2 was at higher levels in a majority of tumor tissues examined, relative to paired normal tissues (PMID: 17626640).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for N4BP2 antibody 23892-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |