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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAAA Polyclonal antibody (16576-1-AP)

    WB analysis using 16576-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16576-1-AP (NAAA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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NAAA Polyclonal antibody

NAAA Polyclonal Antibody for WB, ELISA
Cat No. 16576-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ASAH-like protein, ASAHL, ASAH like protein, Acylsphingosine deacylase NAAA, Acid ceramidase-like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16576-1-AP targets NAAA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAAA fusion protein Ag9861 相同性解析による交差性が予測される生物種
Full Name N-acylethanolamine acid amidase
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC011854
Gene Symbol NAAA
Gene ID (NCBI) 27163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02083
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAAA (N-acylethanolamine acid amidase), also named ASAHL and PLT, is a novel lysosomal hydrolase structurally and functionally similar to acid ceramidase and is mainly involved in the hydrolysis of fatty amides. NAAA is mainly responsible for the degradation of bioactive fatty amides, such as palmitoylethanolamide (PEA), and is involved in lipid metabolism and fatty acid metabolism processes (PMID: 32191459; PMID: 17712833).

Protocols

Product Specific Protocols
WB protocol for NAAA antibody 16576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols